Microfluidic Cell Cycle Analysis of Spread Cells by DAPI Staining

Microfluidic Cell Cycle Analysis of Spread Cells by DAPI Staining "2279

Single-cell cell cycle analysis is an emerging technique that requires detailed exploration of the image analysis process. In this study, we established a microfluidic single-cell cell cycle analysis method that can analyze cells in small numbers and in situ on a microfluidic chip. In addition, factors that influenced the analysis were carefully investigated. U87 or HeLa cells were seeded and a...

Use of DAPI staining for quantitation of cell chemotaxis.

Analysis of the Trypanosoma brucei cell cycle by quantitative DAPI imaging.

Trypanosoma brucei has two DNA compartments: the nucleus and the kinetoplast. DNA replication of these two compartments only partially coincides. Woodward and Gull [Woodward R, Gull K. Timing of nuclear and kinetoplast DNA replication and early morphological events in the cell cycle of Trypanosoma brucei. J Cell Sci 1990;95:49-57] comprehensively studied the relative timing of the replication a...

In Vitro Cell Cycle Synchronization of Sheep Granulosa Cells

Purpose: To investigate the effect of different methods of synchronization on sheep granulosa cell cycle. Materials and Methods: Granulosa cells were aspirated from ovarian follicles and plated in a DMEM medium containing 15% FBS. Upon 70-80% confluency, the medium of the primarycultured as well as the passaged-5 cells were replaced with the medium containing either 0.5% FBS for 24, 48 and 72 h...

Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining

A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells stained by this method are identical to those generated after fixation and RNase digestion. In con...